gastric feeding tube. 1 ml of test

gastric feeding tube. 1 ml of test meal at 36C or 1 ml physiological
serum for C group was administrated via the feeding tube and
recovery was allowed. The rats were returned to their cages until
blood sample extraction at 0 (before administration), 1.5, 3, 4.5,
8, and 12 h after the test meal or physiological serum
administration.
In order to obtain the blood sample, the rats were anesthetized
in the induction chamber and then arranged in surgery position
with anaesthesia flow through a nosecone. The abdominal cavity
was opened and blood was collected from the abdominal aorta
using a K2EDTA vacutainer tube. After blood extraction, rats were
sacrificed by exsanguination, which was verified by perforating
the diaphragm.
All procedures were approved by the Bioethical and Biosafety
Committee of the Faculty of Biological Sciences of Pontificia Universidad Católica de Chile.
2.3. Analytical procedures
2.3.1. Determination of interesterification kinetics through solid fat
content measurements
Since interesterification modifies the melting profile of lipids,
which is unstable until thermodynamic equilibrium is reached
(Idris & Mat Dian, 2005), the solid fat content (SFC) was used as
an indicator of this equilibrium. The SFC of enzymatically interesterified blends was measured by pulsed Nuclear Magnetic Resonance (p-NMR) according to AOCS Official Method Cd 16-81
(1993). Briefly, dry and filtered samples were put into glass tubes
and completely melted (10 min, 60C) and then solidified (0C,
30 min). Thereafter, samples were allowed to melt into a thermostated bathwater at 10.0, 21.1, 26.7, 33.3, and 40.0C for 15 min.
Finally, the SFC was measured in a Bruker Minispec PC120s pNMR analyser (Bruker Analytische Mestechnik, Rheinstetten,
Germany).
2.3.2. FA profile of dietary fats
The fatty acid methyl esters concentration of raw materials and
interesterified blends was determined by conversion into corresponding methyl esters of fatty acids residues followed by GC
(gas chromatograph HP 5890 and a capillar column BPX-70,
50 m, 0.25lm). This was carried out according to AOCS Official
Method Ce 1-62 (1997).
2.3.3. FA profile of plasma lipids
Blood samples were centrifuged at 3000 rpm, 4C for 10 min.
Plasma was separated and stored at18C until the lipids were
isolated. Lipids were extracted using the method described by
Bligh and Dyer (1959), converted into methyl esters using the
method described byMorrison and Smith (1964), and analysed
by GC using a GC Hewlett Packard 7890 (column J and W DB-FFAP,
30 m, 0.25 mm ID, 0.25lm, FID detector).
2.3.4. Statistical analysis
The absorption studies were performed in parallel to reduce differences caused by external factors. The results are expressed as
the mean ± standard error (SEM) of 3 rats in each group. Statistical
significance of differences between groups was determined using
one-way ANOVA followed by the Tukey HSD test. Differences were
considered significant at P< 0.05. Statistical analysis was performed using Statgraphics 5.0 (Manugistic Inc., Oackland, Va.,
USA). Areas under the curve were determined using GraphPad
Prism 5.0 (GraphPad Software, Inc., CA, USA).