soluble hydrolysis products was qua

soluble hydrolysis products was quantified as glucose according to the procedure presented above.


2.4. Characterization of starch fragments from enzymatic hydrolysis by SE–HPLC/Light Scattering

Supernatants from enzymatic treatments (prior to the treat- ment with amyloglucosidase for glucose quantitation) were filtered through a 0.22 m filter, and 0.2 ml of the filtrate were loaded into a HPLC system (515 pump, Waters Co., Milford, MA, USA); UV detec- tor (Dual Absorbance detector 2487, Waters Co., Milford, MA, USA), connected in series to a differential refrattometer (Optilab T-rEX, Wyatt Co., Santa Barbara, CA, USA) and to a Multi Angle Light Scat- tering instrument (DAWN HELEOS, Wyatt Co., Santa Barbara, CA, USA). Polysaccarides were fractionated on a size-exclusion column
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an extrusion temperature of 50 ◦ C. Pasta P3 was produced from

Hasil (Bahasa Indonesia) 1:

hidrolisis larut produk adalah quantified sebagai glukosa menurut prosedur yang disajikan di atas.


2.4. Karakterisasi Pati fragmen dari hidrolisis enzimatik oleh hamburan SE-HPLC/cahaya

Supernatants dari enzimatik perawatan (sebelum memperlakukan-ment dengan amyloglucosidase untuk glukosa kuantisasi) yang filtered melalui filter 0.22 m, dan 0.2 ml filtrate dimuatkan ke sistem HPLC (515 pompa, perairan Co, Milford, MA, USA); UV trojanny-tor (Dual absorbansi detektor 2487, perairan Co, Milford, MA, USA), dihubungkan secara seri refrattometer diferensial (Optilab T-rEX, Co Wyatt, Santa Barbara, CA, USA) dan Multi sudut Light kotoran - tering alat (Fajar HELEOS, Wyatt Co, Santa Barbara, CA, USA). Polysaccarides yang fractionated pada kolom ukuran-pengecualian
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ekstrusi suhu cita 50 C. Pasta P3 dihasilkan dari