Effect of probiotic L. plantarum IS

Effect of probiotic L. plantarum IS-10506 and zinc supplementation on humoral immune response and zinc status of Indonesian pre-school children

A 90-day randomized, double-blind, placebo-controlled, pre-post trial was conducted in four groups of Indonesian children aged 12–24 months: placebo, probiotic, zinc, and a combination of probiotic and zinc (n = 12 per group). Microencapsulated Lactobacillus plantarum IS-10506 of dadih origin was supplemented at a dose of 10 10 CFU/day as a probiotic. Zinc was supplemented as 20 mg zinc sulfate monohydrate (8 mg zinc elemental). Blood and stool samples were collected at baseline and at the end of the study period. Fecal sIgA was assessed by ELISA and serum zinc concentrations by ICP-MS. Fecal sIgA increased significantly in the probiotic group (30.33 ± 3.32 g/g; p < 0.01) and in the combination probiotic and zinc group (27.55 ± 2.28 g/g; p < 0.027), as compared with the placebo group (13.58 ± 2.26 g/g). Changes in
serum zinc concentrations in the combination probiotic and zinc group showed the highest elevation at the end of the study period. A combination of probiotic L. plantarum IS-10506 at a dose of 10 10 CFU/day and 8 mg of elemental zinc supplementation showed a potential ability to improve the zinc status of pre-school children. Taken together, supplementation with the probiotic L. plantarum IS-10506 and zinc for 90 days resulted in a significantly increased humoral immune response, as well as improved zinc status, in young children.

Hasil (Bahasa Indonesia) 2:

Pengaruh probiotik L. plantarum IS-10506 dan suplementasi seng pada respon imun humoral dan status seng anak pra-sekolah Indonesia A 90-hari secara acak, double-blind, placebo-controlled, pra-pasca persidangan dilakukan dalam empat kelompok Indonesia anak usia 12-24 bulan: plasebo, probiotik, seng, dan kombinasi probiotik dan seng (n = 12 per kelompok). Mikroenkapsulasi Lactobacillus plantarum IS-10506 asal dadih dilengkapi dengan dosis 10 10 CFU / hari sebagai probiotik. Zinc dilengkapi dengan 20 mg seng sulfat monohidrat (8 mg seng elemental). Darah dan tinja sampel dikumpulkan pada awal dan pada akhir masa studi. Tinja sIgA dinilai dengan ELISA dan konsentrasi seng serum dengan ICP-MS. Fecal sIgA meningkat secara signifikan pada kelompok probiotik (30,33 ± 3.32 g / g; p <0,01) dan dalam kombinasi probiotik dan seng kelompok (27,55 ± 2,28 g / g; p <0,027), dibandingkan dengan kelompok plasebo (13,58 ± 2.26 g / g). Perubahan konsentrasi seng serum pada kelompok kombinasi probiotik dan seng menunjukkan elevasi tertinggi pada akhir masa studi. Kombinasi probiotik L. plantarum IS-10506 dengan dosis 10 10 CFU / hari dan 8 mg suplemen zinc elemental menunjukkan kemampuan potensial untuk meningkatkan status seng anak pra-sekolah. Secara keseluruhan, suplementasi dengan probiotik L. plantarum IS-10506 dan seng selama 90 hari menghasilkan respon imun humoral meningkat secara signifikan, serta meningkatkan status seng, pada anak-anak.