MATERIALS AND METHODSKetorolac trom

MATERIALS AND METHODS
Ketorolac tromethamine was obtained as a gift sample from Symed
labs; Hyderabad. Gelrite was obtained as gift sample from Sigma
Labs. Acular 0.5% (Mfg by: Allergan) was purchased at local medical
stores Glass cylinders were fabricated at Murthy labs works,
Hyderabad. A.P. Rabbits used for the Draize eye irritancy studies
were from the college animal house and the Goat’s cornea for the exvivo
studies was obtained from the local slaughter house.
Preparation of the formulations
Preparation of insitu
gelling systems
Gelrite of different concentrations of (0.5% w/v, 0.75% w/v and 1%
w/v) was dispersed in deionized water, heated to 90°C while stirring
then cooled to room temperature 13. The formulations were shown
in Table 2.These prepared gels were evaluated for gelling capacity in
simulated tear fluid (pH 7.4). The optimized formula is shown in
Table no 3. The ion activated gels with gelrite were characterized for
clarity, viscosity, assay by UV, pH, FTIR, ocular irritancy and ex‐vivo
corneal studies.

Hasil (Bahasa Indonesia) 2:

BAHAN DAN METODE
Ketorolac trometamin diperoleh sebagai sampel hadiah dari Symed
laboratorium; Hyderabad. Gelrite diperoleh sebagai sampel hadiah dari Sigma
Labs. Acular 0,5% (Mfg oleh: Allergan) dibeli pada kesehatan setempat
toko silinder kaca yang dibuat di laboratorium Murthy bekerja,
Hyderabad. Kelinci AP digunakan untuk studi iritasi mata Draize
berasal dari rumah hewan perguruan tinggi dan kornea kambing untuk exvivo
penelitian diperoleh dari rumah potong hewan setempat.
Penyusunan formulasi
Persiapan insitu
sistem gel
Gelrite konsentrasi berbeda (0,5% w / v, 0,75% b / v dan 1%
b / v) yang tersebar dalam air deionisasi, dipanaskan sampai 90 ° C sedangkan pengadukan
kemudian didinginkan sampai suhu kamar 13. formulasi ditunjukkan
pada Tabel 2.These disusun gel dievaluasi untuk gelling kapasitas di
cairan air mata simulasi (pH 7,4). Rumus dioptimalkan ditunjukkan pada
Tabel ada 3. gel diaktifkan ion dengan gelrite ditandai untuk
kejelasan, viskositas, uji oleh UV, pH, FTIR, iritasi mata dan mantan vivo
studi kornea.