TreatmentOn July 13th, one hundred

Treatment
On July 13th, one hundred and four ewes were treated with an
intravaginal sponge containing 40 mg fluoroprogesterone acetate
(FGA; Intervet Internatinal, Boxmeer, Holland). FGA sponges
were inserted for 12 days into the vagina of ewes, and 500 IU
equine chorionic gonadotropin (eCG, Serotropin; Teikoku Zoki,
Tokyo, Japan) was intramuscularly injected one day before
removal of the FGA sponges.
Chemical reagents
All chemical reagents for preparation of semen extenders were
of the highest purity commercially available. Tris-hydroxymethyl
aminomethane (Tris) was purchased from Merck (Darmstadt, Germany).
Fructose and bovine serum albumin (BSA) were purchased
from Sigma-Aldrich (St. Louis, MO, USA). Citric acid and glycerol
were purchased from Wako (Osaka, Japan). A synthetic
semen extender (AndroMed) was imported from Mini-tub (Tiefenbach,
Germany).

Hasil (Bahasa Indonesia) 2:

Pengobatan
Pada tanggal 13 Juli, seratus empat domba betina diperlakukan dengan
spons intravaginal mengandung 40 mg fluoroprogesterone asetat
(FGA; Intervet Internatinal, Boxmeer, Holland). FGA spons
dimasukkan selama 12 hari ke dalam vagina domba, dan 500 IU
chorionic gonadotropin kuda (EKG, Serotropin, Teikoku Zoki,
Tokyo, Jepang) adalah intramuskuler disuntikkan satu hari sebelum
penghapusan spons FGA.
Kimia reagen
Semua reagen kimia untuk persiapan extender semen adalah
kemurnian tertinggi yang tersedia secara komersial. Tris-hidroksimetil
aminomethane (Tris) dibeli dari Merck (Darmstadt, Jerman).
Fruktosa dan bovine serum albumin (BSA) yang dibeli
dari Sigma-Aldrich (St Louis, MO, USA). Asam sitrat dan gliserol
yang dibeli dari Wako (Osaka, Jepang). Sebuah sintetis
semen extender (AndroMed) didatangkan dari Mini-bak (Tiefenbach,
Jerman).