Uncontrolled diabetes mellitus (DM)

Uncontrolled diabetes mellitus (DM) caused stress oxidative. Oxidative stress was a condition caused by inbalance free radicals and anti oxidant in the human body. Production free radicals increased lipid peroxidation that can produced malondialdehyd (MDA). Exogenous anti oxidant like vitamin C and E are needed to prevent the effects of free radicals on diabetic patients and diabetic complication. This study was aimed to investigate the effects of vitamin C and E supplementation on blood MDA and Cholesterol of alloxan induced-diabetes mellitus rabbits.
The experimental laboratory research conducted 12 rabbits weight of 300-400 gram and age between 2 - 3 months and later divided into 3 groups. The first group was the negative control, second group was the positive control (alloxan induced), third group was treated with vitamin C (7 mg/day) and vitamin E (4.2 IU/day) per oral for 15 days. Data were analyzed using One Way Anova with convidence of degree 99%.
Results showed mean MDA blood level in negative control group was 4.256 ± 0.423 μmol/ml, positive control group was 9.476 ± 0.469 μmol/ml, treated group was 7.327 ± 1.428 μmol/ml. Result showed significant differences in mean of MDA blood levels of rabbits between the 3 groups (p 0.01). From this study can be concluded that vitamin C and E supplementation has potential effect in MDA blood level and but not change to cholesterol blood alloxan induced-diabetes mellitus rabbits.

Hasil (Bahasa Indonesia) 1:

Uncontrolled diabetes mellitus (DM) caused stress oxidative. Oxidative stress was a condition caused by inbalance free radicals and anti oxidant in the human body. Production free radicals increased lipid peroxidation that can produced malondialdehyd (MDA). Exogenous anti oxidant like vitamin C and E are needed to prevent the effects of free radicals on diabetic patients and diabetic complication. This study was aimed to investigate the effects of vitamin C and E supplementation on blood MDA and Cholesterol of alloxan induced-diabetes mellitus rabbits. The experimental laboratory research conducted 12 rabbits weight of 300-400 gram and age between 2 - 3 months and later divided into 3 groups. The first group was the negative control, second group was the positive control (alloxan induced), third group was treated with vitamin C (7 mg/day) and vitamin E (4.2 IU/day) per oral for 15 days. Data were analyzed using One Way Anova with convidence of degree 99%. Results showed mean MDA blood level in negative control group was 4.256 ± 0.423 μmol/ml, positive control group was 9.476 ± 0.469 μmol/ml, treated group was 7.327 ± 1.428 μmol/ml. Result showed significant differences in mean of MDA blood levels of rabbits between the 3 groups (p <0.01) and three was no significant differences in mean cholesterol blood of rabbits between the 3 groups (p> 0.01). From this study can be concluded that vitamin C and E supplementation has potential effect in MDA blood level and but not change to cholesterol blood alloxan induced-diabetes mellitus rabbits.

Hasil (Bahasa Indonesia) 2:

Diabetes mellitus yang tidak terkontrol (DM) yang disebabkan stres oksidatif. Stres oksidatif adalah suatu kondisi yang disebabkan oleh radikal bebas dan anti oksidan ketidakseimbangan dalam tubuh manusia. Produksi radikal bebas meningkat peroksidasi lipid yang dapat dihasilkan malondialdehyd (MDA). Eksogen anti oksidan seperti vitamin C dan E yang dibutuhkan untuk mencegah efek radikal bebas pada pasien diabetes dan komplikasi diabetes. . Penelitian ini bertujuan untuk menyelidiki efek dari vitamin C dan E suplemen di MDA darah dan kolesterol aloksan diinduksi-diabetes mellitus kelinci
Penelitian laboratorium eksperimental dilakukan 12 kelinci berat 300-400 gram dan usia antara 2-3 bulan dan kemudian dibagi menjadi 3 kelompok. Kelompok pertama adalah kontrol negatif, kelompok kedua adalah kontrol positif (aloksan diinduksi), kelompok ketiga diobati dengan vitamin C (7 mg / hari) dan vitamin E (4,2 IU / hari) per oral selama 15 hari. Data dianalisis menggunakan One Way Anova dengan + ingkat kepercayaan dari tingkat 99%.
Hasil penelitian menunjukkan rata-rata tingkat darah MDA pada kelompok kontrol negatif 4,256 ± 0,423 umol / ml, kelompok kontrol positif 9,476 ± 0,469 umol / ml, kelompok perlakuan itu 7,327 ± 1,428 umol / ml. Hasil penelitian menunjukkan perbedaan yang signifikan dalam rerata kadar MDA kelinci antara 3 kelompok (p <0,01) dan tiga tidak ada perbedaan yang signifikan dalam darah kolesterol rata-rata kelinci antara 3 kelompok (p> 0,01). Dari penelitian ini dapat disimpulkan bahwa vitamin C dan E suplemen memiliki efek potensial di tingkat darah MDA dan tetapi tidak mengubah aloksan darah kolesterol diinduksi-diabetes mellitus kelinci.