, cytotoxicity, cancer cell, MCF7,

, cytotoxicity, cancer cell, MCF7, H1299
I. INTRODUCTION
Jacalin is a plant lectin from Artocarpus heterophyllus seed, is a tetrameric two-chain lectin with molecule structure weight is 66kDa. It is combining a heavy α chain and light β chain with of 133 and 20 amino acid residues respectively [1]-[3]. Jacalin is galactose binding lectin type with symbol AIL and ligand motif (Sia)Galβ1- 3GalNAcα1-Ser/Thr [4]. The recovery of more than 50% of the jacalin from crude protein seed extracts which it is single major protein and the resultant product is very expensive because of the extensive purification procedures [5]. However, the abundance of sources material (jackfruit seed) for the production of jacalin has made it an attractive cost-effective lectin, especially in Malaysia. Recent advancement in medicine studies, have been carried out on jacalin revealing its importance as a lectin of diverse applications ranging from the isolation of human IgA and AIDS research [2]. Jacalin also useful tool for studying serum, which is it can be used for the purification of glycoprotein containing O-linked

Manuscript received October 5, 2014; revised December 8, 2014.
2014 Engineering and Technology Publishing doi: 10.12720/jolst.2.2.55-59
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oligosaccharides and as histochemical detection of the Thomsen-Friedenreich antigen tumours.
In the production of jacalin, crude protein is recovered from A. heterophyllus seed by phosphate buffer saline (PBS) precipitation and followed by purification of jacalin. The crude protein contains total protein, thus the purification process is necessary in order to purify the crude seed and recover the purified lectin or jacalin. There are many pharmacological research studies focusing intensively on jacalin now days, however this paper to investigate the antiproliferation effect on MCF7 and H1299 in comparative of crude protein and jacalin extracted from A. heterophyllus seed.
II. MATERIALS AND METHODS