Adult domestic ducks (Cairina mosch

Adult domestic ducks (Cairina moschata) were purchased
from farmers and transported to the Department of Veterinary
Anatomy and Physiology of the University of Nairobi.
The animals were subjected to the selected investigative
procedures as detailed below (outlined in Table 1). In all
cases, anesthesia was achieved by intra-abdominal injection
of Euthatal (sodium pentobarbitone, injected i.p. at a dosage of 50 mg kg−1 bodymass). All protocols were approved by the
Animal Care and Use Committee of the University.
2.1. Tissue Fixation. Lungs intended for microscopy were
fixed in situ by intratracheal infusion with a solution of
2.5% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4,
350 mOsm) and subsequent immersion into the same
fixative.
2.2. Macroscopic Observations. Lungs were carefully dissected
out by cutting off the ribs. After a general observation,
a longitudinal cutwas made on the ventral aspect of the lung
along the long axis of the intrapulmonary primary bronchus,
thus revealing the internal aspect of the mesobronchus.
Identification and enumeration of the various categories of
secondary bronchi emerging fromthe primary bronchus was
conducted. In addition, lungs were subjected to macrophotography
using a digital camera.
2.3. Light and Transmission Electron Microscopy. Fixed
lungs were sliced into slabs and then diced into small
blocks. Tissue blocks were postfixed in osmium tetroxide;
block-stained with uranyl acetate, dehydrated through
ascending concentrations of ethanol, and embedded in
epoxy resin. Semithin sections were obtained at a nominal
thickness of 1

Hasil (Bahasa Indonesia) 1:

Dewasa domestik bebek (Cairina moschata) dibelidari petani dan diangkut ke Departemen hewanAnatomi dan fisiologi dari University of Nairobi.Hewan menjadi sasaran untuk dipilih investigasidijabarkan sebagai rinci di bawah ini (di dalam tabel 1). Dalam semuakasus, anestesi dicapai dengan injeksi intra-abdomendari Euthatal (natrium pentobarbitone, IP disuntikkan pada dosis 50 mg kg−1 bodymass). Semua protokol disetujui olehPenggunaan Komite Universitas dan perawatan hewan.2.1. jaringan fiksasi. Paru-paru yang ditujukan untuk mikroskop yangtetap di situ oleh infus intratracheal dengan larutan2,5% glutaraldehid di 0.1M cacodylate buffer (pH 7,4,350 mOsm) dan selanjutnya pencelupan ke dalam samafiksatif.2.2. makroskopik pengamatan. Paru-paru yang hati-hati membedaholeh memotong tulang rusuk. Setelah pengamatan umum,cutwas longitudinal yang dibuat pada aspek ventral paru-parusepanjang panjang sumbu utama bronkus intrapulmonary,dengan demikian mengungkapkan aspek internal mesobronchus.Identifikasi dan penghitungan berbagai kategoribronchi sekunder yang muncul dari bronkus utama adalahdilakukan. Selain itu, paru-paru yang mengalami macrophotographymenggunakan kamera digital.2.3. cahaya dan mikroskop elektron transmisi. Tetapparu-paru iris lembaran dan kemudian potong dadu kecilblok. Jaringan blok yang postfixed di osmium tetroxide;blok ternoda dengan asetat uranyl, dehidrasi melaluimendaki konsentrasi etanol, dan tertanam dalamresin epoksi. Bagian semithin diperoleh di nominalketebalan 1