tsi (triple sugar iron)agar testaim

tsi (triple sugar iron)agar test
aim: to differentiate among and between the members of enterobacteraceae family
principle:
*study and identify the organisms belonging to enterobacteriaceae family
*it is also used to distinguish the enterobacteriaceae from other gram negative intestinal bacili (by their ability to catabolize glucose, lactose or sucrose and to liberate sulfides from ferrous ammonium sulfate or sodium thiosulfate)
*tsi agar slants contain a 1% concentration of lactose and sucrose, and 0,1% glucose
*the ph indicator, phenol red is also incorporated into the medium to detect acid production from carbohydrate fermentation
*the uninoculated medium is red in colour due to presence of phenol red dye

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tsi (triple sugar iron)agar testaim: to differentiate among and between the members of enterobacteraceae familyprinciple:*study and identify the organisms belonging to enterobacteriaceae family*it is also used to distinguish the enterobacteriaceae from other gram negative intestinal bacili (by their ability to catabolize glucose, lactose or sucrose and to liberate sulfides from ferrous ammonium sulfate or sodium thiosulfate)*tsi agar slants contain a 1% concentration of lactose and sucrose, and 0,1% glucose*the ph indicator, phenol red is also incorporated into the medium to detect acid production from carbohydrate fermentation*the uninoculated medium is red in colour due to presence of phenol red dye

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TSI (triple sugar iron) agar uji
tujuan: untuk membedakan antara dan antara anggota keluarga enterobacteraceae
prinsip:
* belajar dan mengidentifikasi organisme milik keluarga enterobacteriaceae
* juga digunakan untuk membedakan enterobacteriaceae dari gram lainnya bacili usus negatif (dengan mereka kemampuan untuk catabolize glukosa, laktosa atau sukrosa dan untuk membebaskan sulfida dari besi amonium sulfat atau natrium tiosulfat)
* TSI agar miring mengandung konsentrasi 1% laktosa dan sukrosa, glukosa dan% 0,1
* indikator ph, fenol merah juga dimasukkan ke dalam media untuk mendeteksi produksi asam dari fermentasi karbohidrat
* media diinokulasi berwarna merah karena adanya fenol pewarna merah

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