Principle of Separation in Chromato

Principle of Separation in Chromatography

Ion exchange chromatography

• Separation is mainly based on differences in the ion-
exchange affinities of sample components

• A resin (the stationary solid phase) is used to
covalently attach anions or cations onto it

• Solute ions of the opposite charge in the mobile
liquid phase are attracted to the resin by
electrostatic forces

Principle of Separation in Chromatography

Ion exchange chromatography

• Applied commonly in the purification of biological
materials

• Cation exchange : the stationary phase carries a
negative charge, and

• Anion exchange : the stationary phase carries a
positive charge

• Charged molecules in the liquid phase pass through the column until a binding site in the stationary phase appears

Principle of Separation in Chromatography

Ion exchange chromatography

• The molecule of analyte will not elute from the column until a solution with varying pH or ionic strength is passed through it

• Highly selective separation

• Applied early in the overall (separation) process due
to fairly inexpensive and high capacities of resins

Principle of Separation in Chromatography

Ion exchange chromatography

• Separation is mainly based on differences in the ion-
exchange affinities of sample components

• A resin (the stationary solid phase) is used to
covalently attach anions or cations onto it

• Solute ions of the opposite charge in the mobile
liquid phase are attracted to the resin by
electrostatic forces
 
Principle of Separation in Chromatography

Ion exchange chromatography

• Applied commonly in the purification of biological
materials

• Cation exchange : the stationary phase carries a
negative charge, and

• Anion exchange : the stationary phase carries a
positive charge

• Charged molecules in the liquid phase pass through the column until a binding site in the stationary phase appears
 
Principle of Separation in Chromatography

Ion exchange chromatography

• The molecule of analyte will not elute from the column until a solution with varying pH or ionic strength is passed through it

• Highly selective separation

• Applied early in the overall (separation) process due
to fairly inexpensive and high capacities of resins

0/5000

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Prinsip pemisahan di kromatografi

kromatografi pertukaran Ion

• pemisahan adalah terutama didasarkan pada perbedaan dalam ion-
pertukaran afinitas contoh komponen

• resin (fase padat stasioner) digunakan untuk
berikatan kovalen dengan melampirkan anion atau kation ke atasnya

ion solut • charge berlawanan di ponsel
fase cair yang tertarik resin oleh
elektrostatik pasukan

Prinsip pemisahan di kromatografi

kromatografi pertukaran Ion

• Applied lazim dalam pemurnian biologis
bahan

• tukar kation: fase stasioner membawa
negatif biaya, dan

• Anion Asing: fase stasioner membawa
muatan positif

• Dikenakan molekul dalam fase cair melewati kolom sampai muncul sebuah situs mengikat dalam fase stasioner

prinsip pemisahan di kromatografi

kromatografi pertukaran Ion

• molekul analyte akan tidak elute dari kolom sampai solusi dengan pH yang bervariasi atau kekuatan ionik melewatinya

• pemisahan sangat selektif

• Diterapkan pada awal proses (pemisahan) secara keseluruhan karena
untuk kapasitas yang cukup murah dan tinggi resin

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Prinsip Pemisahan di Kromatografi Ion kromatografi pertukaran • Pemisahan terutama didasarkan pada perbedaan ion- afinitas pertukaran komponen sampel • Sebuah resin (fase padat stasioner) digunakan untuk kovalen melampirkan anion atau kation ke atasnya • ion terlarut dari muatan yang berlawanan dalam ponsel fase cair tertarik pada resin dengan gaya elektrostatik Prinsip Pemisahan di Kromatografi kromatografi pertukaran ion • Berlaku umum dalam pemurnian biologis bahan • pertukaran kation: fase diam membawa muatan negatif, dan pertukaran • Anion: fase diam membawa muatan positif • Dibebankan molekul dalam fase cair melewati kolom sampai situs pengikatan dalam fase diam muncul Prinsip Pemisahan di Kromatografi kromatografi pertukaran ion • Molekul analit tidak akan terelusi dari kolom sampai solusi dengan berbagai pH atau kekuatan ion melewatinya • pemisahan Sangat selektif • Diterapkan di awal keseluruhan (pemisahan) proses karena untuk kapasitas cukup murah dan tinggi resin

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